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pENTR4-EGFP_opt

Plasmid

#106334

Purpose

Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

EGFP

Use

Gateway entry vector

Tags

Expression

Bacterial and Mammalian

Mutation

codon optimized for gene synthesis without affect…

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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PL-452 C-EGFP

Plasmid

#19178

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

EGFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLTE EGFP

Plasmid

#67044

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EGFP

Use

Cell-free expression

Tags

Myc

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
8024 GFP_Kras_G12C_2A_puro

Plasmid

#64373

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2A (Kras Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

changed glycine 12 to cysteine

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP TEM4 FL

Plasmid

#58893

Purpose

Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

Depositor

Channing Der

Article

Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

Insert

TEM4 (ARHGEF17 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L722F

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-CHAMP1

Plasmid

#159892

Purpose

Expresses CHAMP1 (CAMP) in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

Insert

CHAMP1 (CHAMP1 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Tubulin-LSSmGFP

Plasmid

#186535

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Use

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 WT

Plasmid

#26726

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

Rabin8 (RAB3IP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pJUMP27-1A(sfGFP)

Plasmid

#126974

Purpose

Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Lifeact_Halo_T2A_EGFP

Plasmid

#135445

Purpose

Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

Lifact-HaloTag7 and EGFP

Use

Tags

Lifact

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-rSyntaxin-3a

Plasmid

#195206

Purpose

Mammalian expression vector for GFP tagged Syntaxin-3a.

Depositor

Thomas Weimbs, David Bryant

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

STX3A (Stx3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-BICD1

Plasmid

#49487

Purpose

expresses EGFP-BICD1 in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu

Insert

BICD1 (BICD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

** see notes

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
sfGFP-PMP-N-10

Plasmid

#56295

Purpose

Localization: Peroxisomes, Excitation: 485, Emission: 507

Depositor

Michael Davidson

Insert

PMP (PXMP2 Human)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Let-7b-GFP

Plasmid

#25404

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

Mirlet7b (Mirlet7b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hUVRAG

Plasmid

#24296

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pATZ-HA-GFP11

Plasmid

#177720

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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