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pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-8

Plasmid

#228990

Purpose

For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-8

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_2001-3000

Plasmid

#198586

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_2001-3000

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHM148

Plasmid

#225532

Purpose

Encodes constitutive rtTA, tetOn-Pcdh19-GFP, and constitutive mCherry

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
Pcdh19 (Pcdh19 Mouse)
mCherry

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225645

Purpose

tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA and LacO3O1 in tandem conformation

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA and LacO3O1 promoters

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225648

Purpose

LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1 and tetR/tetA in tandem conformation

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 and tetA promoters

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225647

Purpose

LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1 and tetR/tetA in tandem conformation

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 and tetA promoters

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225646

Purpose

tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA and LacO3O1 in tandem conformation

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA and LacO3O1 promoters

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BPLF1_1-1000

Plasmid

#198582

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BPLF1_1-1000

Use

Gateway entry vector

Tags

Expression

Mutation

None (wildtype)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP-IFP2.0C

Plasmid

#215752

Purpose

Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xCBX7CD-IFP2.0C

Plasmid

#215754

Purpose

Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD-IFP2.0C

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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RGG-BcLOV-mCh-EGFR

Plasmid

#208280

Purpose

Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

RGG-BcLOV-mCh-EGFR

Use

Lentiviral

Tags

Expression

Mutation

H225N in mCherry- please see depositor comment

Promoter

pCMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 6X PRE

Plasmid

#206168

Purpose

pMuLE L1-L4 entry plasmid containing 6 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

6X PRE

Use

Gateway cloning entry clone

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 4X PRE

Plasmid

#206167

Purpose

pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE

Use

Gateway cloning entry clone

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge