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pCR3.1-OPNc PSM4

Plasmid

#106329

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M5

Plasmid

#106325

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc splice site insertion mutant EEKQNA-->EEK…

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M3

Plasmid

#106323

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

OPNc mutated splice site EEKQNAV-->AAAAAAA

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1

Plasmid

#81227

Purpose

Hybrid Tar[1-43]-McpC[49-270]-Tar[184-553] expression plasmid

Depositor

Victor Sourjik

Article

Bi et al ACS Synth Biol. 2016 Jun 10.

Insert

Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: Tar[1-43]-McpC[49-270]-Tar[184-553]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB3647

Plasmid

#80981

Purpose

Used for abrB3647(CAC3647) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB3647(CAC3647)

Use

Tags

Expression

Bacterial

Mutation

Promoter

thl (cac2873)

Available since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB1941

Plasmid

#80980

Purpose

Used for abrB1941(CAC1941) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB1941(CAC1941)

Use

Tags

Expression

Bacterial

Mutation

Promoter

thl (cac2873)

Available since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pIMP1-Pthl-AbrB0310

Plasmid

#80979

Purpose

Used for abrB0310(CAC0310) overexpression in Clostridium acetobutylicum

Depositor

Wei-Hong Jiang

Article

Xue et al Appl Microbiol Biotechnol. 2016 Jun 8.

Insert

abrB0310(CAC0310)

Use

Tags

Expression

Bacterial

Mutation

Promoter

thl (cac2873)

Available since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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SAH0141-0.0-conP01

Plasmid

#79828

Purpose

Transcriptional interference assessment; strong interfering promoter, landing site for spacers

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and conP01

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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SAH0146-0.5-conP01

Plasmid

#79831

Purpose

Transcriptional interference assessment; strong interfering promoter, 500 bp spacer

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and conP01

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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SAH0098-scrPX

Plasmid

#79830

Purpose

Transcriptional interference assessment; no interfering promoter (scrambled)

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and scrPX (no promoter activity)

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-Mb-crRNA

Plasmid

#78959

Purpose

Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AN+

Plasmid

#78240

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS-

Plasmid

#78239

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS+

Plasmid

#78236

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS+

Plasmid

#78252

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCVD432

Plasmid

#63755

Purpose

Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.

Depositor

James Kaper

Article

Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.

Insert

EAggEC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
mRFP1-19

Plasmid

#63820

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP1-35

Plasmid

#63824

Purpose

A member of an RBS library for mRFP1 with different translation rates

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

J23100

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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