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Plasmid#65559PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
sTR056
Plasmid#177369PurposeToehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTR060
Plasmid#177370PurposeTrigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.DepositorInsertTrigger RNA
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Tbx5
Plasmid#72688PurposeDoxycyline regulated expression of Tbx5 in mammalian cellsDepositorInsertTbx 5 (Tbx5 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CNOT4_RRM-ZNF_pGEX
Plasmid#135146PurposeRecombinant expression of CNOT4 with dual-affinity tagDepositorInsertCNOT4 (CNOT4 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[85Q]-S776A
Plasmid#33240DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsflagExpressionMammalianMutationinsert contains a stretch of 85 Glutamines and no…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM5-Flag
Plasmid#180410Purposelentiviral transduction of human TGM5 (with a C-terminal Flag tag) geneDepositorInsertTGM5 (TGM5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1084
Plasmid#24383DepositorInsertCLASP2 (340-1084) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650
Plasmid#24409DepositorInsertCLASP2 (512-650) (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant with only MT plus end trac…PromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorInsertFtnA (ftnA Escherichia coli)
UseTagsBC2-tag and YpetExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-VIM-BC2T
Plasmid#108966Purposeexpresses mCherry-vimentin-BC2-tag in mammalian cellsDepositorInsertVIM (VIM Human)
UseTagsBC2T and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(2Q)
Plasmid#21752DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
DNMT1 KO Neo
Plasmid#16649DepositorInsertDNMT1 knock-out construct (DNMT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA1
Plasmid#113130PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 for gRNA expression, RSV for GFP expressionAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
BOLL_RRM_pGEX
Plasmid#135130PurposeRecombinant expression of BOLL with dual-affinity tagDepositorInsertBOLL (BOLL Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1d834-1363-Poly(A)
Plasmid#65560PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationdel(834-1363)PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMGF157
Plasmid#96972Purposeish1-mCherry insertion cassette with Hygro resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + hygro…PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-neo-C11orf51-3xFlag (2765)
Plasmid#39859DepositorInsertC11orf51 (ANAPC15 Human)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-chCARex
Plasmid#87233Purposebacterial expression of GST-CAR extracellular domainDepositorInsertCXADR (CXADR Chicken)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24523DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJuly 22, 2010AvailabilityAcademic Institutions and Nonprofits only