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Plasmid#191284PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_WTTS
Plasmid#191285PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_WTTS
Plasmid#191286PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_WTTS
Plasmid#191287PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_Q33STS
Plasmid#191288PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_Q33STS
Plasmid#191290PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_Q33STS
Plasmid#191291PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_typeEGF_gRNA
Plasmid#199386PurposepDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNADepositorInsertnrg1 EGF sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK157
Plasmid#200151PurposeType 2 part for Modular Cloning in YeastDepositorInsertpSP6_Kozak
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK159
Plasmid#200153PurposeType 4 part for Modular Cloning in YeastDepositorInserttT7
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK109
Plasmid#200135PurposeType 4a part for Modular Cloning in YeastDepositorInsertBPSV40-NLS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK110
Plasmid#200141PurposeType 3a part for Modular Cloning in YeastDepositorInsertsfGFP11
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK108
Plasmid#200134PurposeType 3a part for Modular Cloning in YeastDepositorInsertBPSV40-NLS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK111
Plasmid#200142PurposeType 4a part for Modular Cloning in YeastDepositorInsertsfGFP11
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK188
Plasmid#200156PurposeType 4a part for Modular Cloning in YeastDepositorInsert6xHis
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK158
Plasmid#200152PurposeType 2 part for Modular Cloning in YeastDepositorInsertpT7_LacO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK115
Plasmid#200148PurposeType 3a part for Modular Cloning in YeastDepositorInsertymNeonGreen
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK161
Plasmid#200155PurposeType 67 part for Modular Cloning in YeastDepositorInsertLacI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK121
Plasmid#200145PurposeType 3 part for Modular Cloning in YeastDepositorInsertsfGFP1-10
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK112
Plasmid#200143PurposeType 3a part for Modular Cloning in YeastDepositorInsertsfGFP1-10
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only