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Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-blue
Plasmid#223532PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-Green
Plasmid#223440PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)
Plasmid#221746PurposeStable expression of GFP-CERT(PHD)in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR77-DuET
Plasmid#213291PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR77 (C5AR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
F2RL1-DuET
Plasmid#213235PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertF2RL1 (F2RL1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-GST-TEV-mCherry-NAP1 (WT)
Plasmid#198036PurposeUsed for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)DepositorInsertNAP1 (AZI2 Human)
UseTagsGST-TEV and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(T59/T61A)(HA)
Plasmid#195025PurposeGateway vector containing HA-tagged Creb5 with T59/T61A mutationDepositorInsertHA-tagged Creb5Â (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationT59/T61APromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Creb5(T59/T61D)(HA)
Plasmid#195026PurposeGateway vector containing HA-tagged Creb5 with T59/T61D mutationDepositorInsertHA-tagged Creb5Â (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
UseGateway cloningTags3xHAExpressionMutationT59/T61DPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Creb5(HA)
Plasmid#195024PurposeHA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vectorDepositorInsertHA-tagged Creb5Â (full length, 508aa) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(T59/T61A)(HA)
Plasmid#195027PurposeHA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5Â (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer20-iCreb5(T59/T61D)(HA)
Plasmid#195028PurposeHA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vectorDepositorInsertHA-tagged Creb5Â (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
UseLentiviralTags3xHAExpressionMammalianMutationT59/T61DPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(VENTX_5-5)-PGKpuroBFP-W
Plasmid#211993PurposeExpress gRNA against VENTX with puro and BFPDepositorInsertsgRNA targeting VENTX (VENTX Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(VENTX_5-4)-PGKpuroBFP-W
Plasmid#211992PurposeExpress gRNA against VENTX with puro and BFPDepositorInsertsgRNA targeting VENTX (VENTX Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 R57A, R59A pMT2
Plasmid#206112Purposeexpression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulationDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mNeon-ACTB
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mNeon-ACTB
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-ACTB
Plasmid#207750PurposeDonor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Solo-mNeon-ACTB
Plasmid#207749PurposeDonor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a mNeon Tag (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CHMP2A-myc, L216D/L219D
Plasmid#180645PurposeMammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsMycExpressionMammalianMutationL216D, L219DPromoterpCAGAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only