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Plasmid#121529PurposeFor making C-terminal mOrange fusions using multisite Gateway cloningDepositorInsertmOrange
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM10-V5-TurboID-GW
Plasmid#196285PurposeGateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic fliesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM10-GW-TurboID-V5
Plasmid#196283PurposeGateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic fliesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
sCFP in pBSDONR P4r-P2
Plasmid#121530PurposeFor making C-terminal sCFP fusions using multisite Gateway cloningDepositorInsertsCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroQ-Cre-ERT2
Plasmid#59701Purposeto make Cre-ERT2 expressing stable cell line by retrovirusDepositorInsertCre-ERT2
UseRetroviralTagsEstrogen receptor (ERT)ExpressionMammalianMutationPromotercmvIEAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
mouse ptc probe M2-3
Plasmid#58701Purposemakes mouse ptc probe for in situ hybridizationDepositorInsertptc (Ptch1 Mouse)
UseIn situ probeTagsExpressionBacterialMutationonly EcoRI fragment (5' end)PromoterT7Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sperm whale Mb 64Y68F in pVP80K
Plasmid#86956PurposeIncreases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).DepositorInsertMb 64Y68F
UseTagsExpressionBacterialMutation64Y68FPromoterT5Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W
Plasmid#32522DepositorInsertdominant negative Rheb (D60K) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationD60K to make dominant negativePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRosa26PAm1 (DM#272)
Plasmid#15036DepositorInsertpRosa26 modified targeting vector
UseFor making transgenic constructsTagsExpressionMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W
Plasmid#32521DepositorInsertDominant negative Rheb (D60I) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationD60I to make dominant negativePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG1guide_RUBY_EggCas9
Plasmid#225983PurposeTo make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plantsDepositorInsertGuide RNA against AtAGAMOUS1
UseCRISPRTagsExpressionPlantMutationPromoter35s promoter to Drive RUBY and DD45p to drive Cas9Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2-M1054V
Plasmid#138538PurposeFor making TNKS-2-M1054V RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationChanged methionine 1054 to valinePromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
C035
Plasmid#174341PurposeTemplate for making Rluc mRNA reporter with pA120.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C034
Plasmid#174340PurposeTemplate for making Rluc mRNA reporter with pA98.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
C255
Plasmid#174352PurposeTemplate for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytesDepositorInserteGFP
UseIn vitro transcription templateTagsFLAG and HAExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
C033
Plasmid#174339PurposeTemplate for making Rluc mRNA reporter with pA63.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C031
Plasmid#174338PurposeTemplate for making Rluc mRNA reporter with pA30.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only