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Addgene

pRosa26PAm1 (DM#272)
(Plasmid #15036)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15036 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRosa26 PA
  • Backbone size w/o insert (bp) 9896
  • Vector type
    for making transgenic constructs

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pRosa26 modified targeting vector

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pRosa26PA from Frank Costantini.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modification of plasmid pRosa26PA described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. See BMC Dev Biol article for more information.

Clone into PacI & AscI sites. The modified plasmid conatins additional sites for linearization. See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRosa26PAm1 (DM#272) was a gift from Douglas Melton (Addgene plasmid # 15036 ; http://n2t.net/addgene:15036 ; RRID:Addgene_15036)
  • For your References section:

    Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333