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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRosa26 PA
- Backbone size w/o insert (bp) 9896
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Vector typefor making transgenic constructs
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepRosa26 modified targeting vector
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypRosa26PA from Frank Costantini.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Modification of plasmid pRosa26PA described in Srinivas et al. BMC Dev Biol (2001) vol 1, p.4. See BMC Dev Biol article for more information.
Clone into PacI & AscI sites. The modified plasmid conatins additional sites for linearization. See Author's Map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRosa26PAm1 (DM#272) was a gift from Douglas Melton (Addgene plasmid # 15036 ; http://n2t.net/addgene:15036 ; RRID:Addgene_15036) -
For your References section:
Notch signaling controls multiple steps of pancreatic differentiation. Murtaugh LC, Stanger BZ, Kwan KM, Melton DA. Proc Natl Acad Sci U S A. 2003 Dec 9. 100(25):14920-5. 10.1073/pnas.2436557100 PubMed 14657333