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nuc-yHS1-M7A,H102A

Plasmid

#159168

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A,H102A

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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mito-yHS1-M7A,H102A

Plasmid

#159165

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A,H102A

Use

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX FynT-SH2

Plasmid

#46430

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynB/FynT (FYN Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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Opto-GPR37L1

Plasmid

#106020

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR37L1 (GPR37L1 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Use

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry KNL1 wt hardened and RVSF->AAAA

Plasmid

#45226

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Use

Tags

TEV S15

Expression

Mutation

RVSF 58 AAAA

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
mIFP12-Actin-C-18

Plasmid

#56244

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Actin (ACTB Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Actin-C-18

Plasmid

#56211

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001101.3 (ACTB Human)

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHR-mCherry-p2A-LIC1 G domain

Plasmid

#74602

Purpose

expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-V159N

Plasmid

#118383

Purpose

Gateway compatible donor vector with actin-V159N.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

V159N

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

G168D,Y169D

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NMRK2 gRNA (BRDN0001145387)

Plasmid

#77130

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK2 (NMRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Use

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Tags

N-terminal GST and Strep II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TTK gRNA (BRDN0001145152)

Plasmid

#75579

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TTK gRNA (BRDN0001146504)

Plasmid

#75580

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TTK gRNA (BRDN0001147779)

Plasmid

#75581

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TTK (TTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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