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pGEX-4T-3-mR7BD

Plasmid

#79149

Purpose

bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

Depositor

Aimee Edinger

Article

Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

Insert

R7BD (Rilp Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 220-299

Promoter

tac

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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ChromosomesMembranesandCytoskeleton

Plasmid

#62450

Purpose

MXS_chaining vector with 4 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::H2B-TagBFPx3-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::mCherryx3-linker-betaActin-bGHpA
CMV::Citrinex3-linker-alphaTubulin-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hRubicon

Plasmid

#64147

Purpose

For protein expression in E. coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Use

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
(SIM)10

Plasmid

#126946

Purpose

Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Use

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCB163

Plasmid

#37388

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Dynactin 2 (DCTN2 Human)

Use

Retroviral

Tags

GFP, S-tag, and TEV

Expression

Mammalian

Mutation

A7P

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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BICDN-CIBN

Plasmid

#102253

Purpose

Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

BICDN-CIB1 (CIB1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Use

Tags

V5

Expression

Bacterial and Insect

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-ER-mTagBFP2

Plasmid

#216127

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)1

Plasmid

#126954

Purpose

Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)3

Plasmid

#126955

Purpose

Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-CIBN-CAAX

Plasmid

#188989

Purpose

A lentiviral plasmid encoding plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

CAAX

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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CIB81-pmGFP

Plasmid

#75366

Purpose

Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Use

Tags

prenylated EGFP

Expression

Mammalian

Mutation

residues 1-81 of CIB1

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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RIPR-bio-His

Plasmid

#50824

Purpose

Expresses enzymatically monobiotinylated full length RIPR

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

RIPR

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Consensus

Plasmid

#176664

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge