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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAc5/V5-HIS
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Backbone manufacturerInvitrogen
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Vector typeInsect Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlag-mCherry-T2A-GFP-T2A-neo
- Promoter Actin5C
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer AC5_PRIMER
- 3′ sequencing primer EBVrev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For multicistronic expression in insect cells.
FLAG-mCherry, GFP, or Neo can be removed or replaced.
N-terminal or C-terminal fusions to either FLAG-mCherry or GFP are possible.
Note: mCherry DNA sequence is modified from original Tsien sequence (AA sequence is unchanged). AA sequence of T2A peptides is identical, but DNA sequence differs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ac5-STABLE2-neo was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 32426 ; http://n2t.net/addgene:32426 ; RRID:Addgene_32426) -
For your References section:
Generation of stable Drosophila cell lines using multicistronic vectors. Gonzalez M, Martin-Ruiz I, Jimenez S, Pirone L, Barrio R, Sutherland JD. Sci Rep. 2011;1:75. doi: 10.1038/srep00075. Epub 2011 Aug 31. 10.1038/srep00075 PubMed 22355594