-
Plasmid#27296DepositorInsertAMP Acticated Protein Kinase (Prkaa2 Rat)
UseRetroviralTagsMycExpressionMammalianMutationKinase Dead = Lys 45 to ArgPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST
Plasmid#216341PurposeFor testing localization of pFAST protein to Golgi complexDepositorInsertST6Gal1_pFAST
UseTagsmyc for FAST proteinExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST
Plasmid#216342PurposeFor testing localization of pFAST protein to mitochondriaDepositorInsertCox8_pFAST
UseTagsmyc for FAST proteinExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-FAST
Plasmid#216340PurposeFor testing localization of pFAST protein to endoplasmic reticulumDepositorInsertPRL_pFAST
UseTagsmyc for FAST proteinExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIP 227 CoMiP 4in1 wo shRNA p53
Plasmid#63727PurposeTo express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMS
Plasmid#51543PurposeInducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2DepositorUseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iM
Plasmid#58973PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-GG-OMKSL-PGK-Puro
Plasmid#102894PurposePiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaster3
Plasmid#58526PurposeReprogram somatic cells to iPS cellsDepositorInsertsUseTagsExpressionMutationPromoterAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OMKSL-PP
Plasmid#102899PurposeEBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMPK alpha2 delta312X
Plasmid#60127Purposeexpresses constitutively active AMPKalpha2DepositorInsertAMPK alpha 2 (Prkaa2 Rat)
UseTagsMycExpressionMammalianMutationTruncated at residue 312PromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-GFP-SPOP (R354H)
Plasmid#197010PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorInsertSPOP (Spop Mouse)
UseTagsGFPExpressionMammalianMutationR354HPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/TO-GFP-SPOP (R221C)
Plasmid#197009PurposeMammalian inducible expression vector for GFP-tagged SPOPDepositorInsertSPOP (Spop Mouse)
UseTagsGFPExpressionMammalianMutationR221CPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SnFR_S074
Plasmid#182811PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertS074-CholineSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SnFR_S062
Plasmid#182810PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertS062-CholineSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisFlag-rUSP19CSdomains
Plasmid#155240Purposefor mammalian overexpression of the CS domains of rat USP19DepositorInsertUSP19 (CS domains) (Usp19 Rat)
UseTags6XHis-3XFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV Tor1a-V5
Plasmid#175135PurposeLentiviral expression of Tor1a-MycDepositorInsertTor1a (Tor1a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby-Endo-14
Plasmid#55859PurposeLocalization: Endosomes, Excitation: 558, Emission: 605DepositorInsertEndo (RHOB Human)
UseTagsmRuby and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
UseTags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -