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Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD3
Plasmid#33106DepositorInsertTEAD3 (TEAD3 Human)
UseTagsGal4ExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AlaRS
Plasmid#124103PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAlanyl-tRNA ligase (alaS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSDest
Plasmid#22423DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149198)
Plasmid#80248Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-p53-shRNA-941
Plasmid#25637DepositorInsertp53-shRNA-941 (TP53 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133APromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERN
Plasmid#80476PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsGFPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B19L
Plasmid#59922PurposeExpression vector for Rabies virus SAD B19 RNA dependent RNA polymerase geneDepositorInsertRabies virus RNA dependent RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-V5-AP-nes_pLX304
Plasmid#120912PurposeLentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosolDepositorInsertFKBP-V5-AP-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP
Plasmid#89684PurposepCAG promoter driving EGFPDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro 3xFLAG Dna2 wt
Plasmid#31955DepositorInserthDna2 (DNA2 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationChanged start ATG to AAA,PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pN1/G3BP1-iRFP
Plasmid#129339PurposeStress granule markerDepositorInsertiRFP (from Dr. M. Matsuda)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -