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Plasmid#193652PurposeExpression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130FPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasWT/sgKras/Cre
Plasmid#99848PurposeExpresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-N57G
Plasmid#193639PurposeExpression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3A-N57G, EGFP
UseTagsExpressionMammalianMutationhAPOBEC3A-N57GPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas12a-A3A-Y130F
Plasmid#193640PurposeExpression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationhAPOBEC3A-Y130FPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R]
Plasmid#114507PurposeMammalian Expression Plasmid of anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Human). Derived from hybridoma N120A/9.1.DepositorInsertanti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Homo sapiens) recombinant mouse monoclonal antibody (NRG1 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel (Mouse) [L6/23R]
Plasmid#128634PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/23.DepositorInsertanti-Slo1/BKAlpha maxi-K+ channel (Mouse) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v2
Plasmid#138119PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoterDepositorInsertMb2Cas12a v2
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172R, N563R and K569RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-Copper ATPase 1 (Menke's disease protein) [L60/4R]
Plasmid#114502PurposeMammalian Expression Plasmid of anti-Copper ATPase 1 (Menke's disease protein) (Human). Derived from hybridoma L60/4.DepositorInsertanti-Copper ATPase 1 (Menke's disease protein) (Homo sapiens) recombinant mouse monoclonal antibody (ATP7A Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v1
Plasmid#138118PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoterDepositorInsertMb2Cas12a v1
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ223-STU
Plasmid#138109PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminatorDepositorInsertdAsCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEE
Plasmid#159746PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RVR-A3A-Y130F
Plasmid#193658PurposeExpression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRVR-dLbCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130FPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-enCas12a-A3A-N57G
Plasmid#193651PurposeExpression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLb-enCas12a-hA3A-N57G, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57GPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RVR-A3A-N57G
Plasmid#193657PurposeExpression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRVR-dLbCas12a-hA3A-N57G, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57GPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-A3A-N57G
Plasmid#193654PurposeExpression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRR-dLbCas12a-hA3A-N57G, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, hAPOBEC3A-N57GPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-A3A-Y130F
Plasmid#193655PurposeExpression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRR-dLbCas12a-hA3A-Y130F, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, hAPOBEC3A-Y130FPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgBrip1#2/Cre
Plasmid#193204PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 geneDepositorInsertsgBrip1#2 (Brip1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only