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Addgene
Showing: 2581 - 2600 of 2912 results

  7. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  8. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

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  9. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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  11. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

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  18. (SIM)5

    Plasmid
    #126947
    Purpose
    Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    unknown
    Available since
    Sept. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126947-plasmid-map-sequence-id-255788

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Showing: 2581 - 2600 of 2912 results