Skip to main content
Addgene
Showing: 5161 - 5200 of 148587 results

  2. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

    Enlarge

  4. pUCsRNAP

    Plasmid
    #182746
    Purpose
    Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids
    Depositor
    James Collins
    Article
    Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d
    Insert
    small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182746-plasmid-map-sequence-id-358079

    Enlarge

  6. pGP-AAV-syn-iGABASnFR2n-WPRE

    Plasmid
    #218875
    Purpose
    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)
    Depositor
    GENIE Project
    Insert
    iGABASnFR2n
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S99A F104H R168P
    Promoter
    Synapsin
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218875-plasmid-map-sequence-id-433406

    Enlarge

  13. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR T757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

    Enlarge

  24. pLV6-Bmal-luc

    Plasmid
    #68833
    Purpose
    Expression of Bmal1-luc in mammalian cells
    Depositor
    Steven Brown
    Insert
    Bmal1 regulatory sequences (Bmal1 Mouse)
    Use
    Lentiviral and Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68833-plasmid-map-sequence-id-121399

    Enlarge

  27. pTwist GFP-Linker-VDAC1

    Plasmid
    #211735
    Purpose
    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.
    Depositor
    Roland Malli
    Article
    Pilic et al bioRxiv 2023.09.08.556841
    Insert
    Voltage-dependent anion channel 1 (VDAC1 )
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211735-plasmid-map-sequence-id-424070

    Enlarge

  28. RP4-7

    Plasmid
    #79811
    Purpose
    Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Chloramphenicol acetyltransferase
    Use
    Helper plasmid for mobilization
    Tags
    Expression
    Mutation
    Promoter
    Own promoter
    Available since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. TFORF2956

    Plasmid
    #144432
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144432-plasmid-map-sequence-id-407321

    Enlarge

  30. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

    Enlarge

  31. pSB700

    Plasmid
    #64046
    Purpose
    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
    Depositor
    George Church
    Article
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CAGGS
    Available since
    May 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64046-plasmid-map-sequence-id-313670

    Enlarge

Showing: 5161 - 5200 of 148587 results