Addgene

Showing: 2581 - 2600 of 2721 results

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pYPQ284-RVRR

Plasmid

#160327

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a RVRR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N563R, K569V, N573R and K625R

Promoter

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

Plasmid

#170228

Purpose

Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Fusion

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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Anti-LRRK2/Dardarin, N-terminus [N138/6R]

Plasmid

#182104

Purpose

Mammalian Expression Plasmid of anti-LRRK2/Dardarin, N-terminus (Human). Derived from hybridoma N138/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-LRRK2/Dardarin, N-terminus (Homo sapiens) recombinant mouse monoclonal antibody (LRRK2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Use

Tags

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y130F

Plasmid

#193640

Purpose

Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

hAPOBEC3A-Y130F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-N57G

Plasmid

#193651

Purpose

Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-N57G, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-ABE8e-V106W

Plasmid

#193653

Purpose

Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-RR-A3A-N57G

Plasmid

#193654

Purpose

Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RR-dLbCas12a-hA3A-N57G, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, hAPOBEC3A-N57G

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RR-A3A-Y130F

Plasmid

#193655

Purpose

Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RR-dLbCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, hAPOBEC3A-Y130F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-RR-ABE8e-V106W

Plasmid

#193656

Purpose

Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K595R, TadA*8e-V106W

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-RVR-A3A-N57G

Plasmid

#193657

Purpose

Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-hA3A-N57G, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RVR-A3A-Y130F

Plasmid

#193658

Purpose

Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-RVR-ABE8e-V106W

Plasmid

#193659

Purpose

Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

RVR-dLbCas12a-TadA*8e-V106W, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Lenti-sgApc#2/Cre

Plasmid

#193202

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Apc gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgApc#2 (Apc Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Lenti-sgBrip1#1/Cre

Plasmid

#193203

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgBrip1#1 (Brip1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgBrip1#2/Cre

Plasmid

#193204

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Brip1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgBrip1#2 (Brip1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCol11a1#1/Cre

Plasmid

#193205

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Col11a1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCol11a1#1 (Col11a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge