-
Plasmid#124544PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55A, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124540PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdelta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA
Plasmid#124538PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41PromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAdam10 v3
Plasmid#158034Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10DepositorInsertAdam10 sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLG1-puro-sgATL1-5
Plasmid#109007PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #5 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA
Plasmid#124537PurposeCloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41 C55GPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2
Plasmid#71348PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgUroD v4
Plasmid#158036Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroDDepositorInsertUroD sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-Cre sgItgb5 v3
Plasmid#158038Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5DepositorInsertItgb5 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-Cre sgRipk4 v3
Plasmid#158035Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4DepositorInsertRipk4 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLG1-puro-sgATL1-2
Plasmid#109006PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #2 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-shP53
Plasmid#124261PurposeExpresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshP53
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2-shATRX
Plasmid#124258PurposeExpresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshATRX
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAX198
Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
KCC2-pHext
Plasmid#104076PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagspHluorine tag inserted in the second putative tra…ExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1290
Plasmid#119267Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335-NQL002-WAPL-sgRNA1
Plasmid#175550PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.DepositorInsertspCas9-nickase and sgRNA against mouse WAPL STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX335-NQL003-WAPL-sgRNA2
Plasmid#175551PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.DepositorInsertspCas9-nickase and sgRNA against mouse WAPL STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only