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Plasmid#182547PurposeExpresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificityDepositorInsertH5_Vietnam_1203_04_Y161A
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationY161A mutant that confers exclusive NeuAc to NeuG…PromoterCMVAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mT2A-td-sfGFP-FNF
Plasmid#182339PurposeRecombineering cassette plasmid for T2A-td-sfGFP-FNFDepositorInsertT2A-td-sfGFP-FNF
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen and J23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G
Plasmid#169315PurposeDoxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent proteinDepositorInsertmiR-125b-2 (MIR125B2 Human)
UseLentiviralTagsdTomatoExpressionMutationPromoterTREAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v3-sfGFP
Plasmid#145174PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 3DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVY-3 sfGFP-tag3
Plasmid#194618Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertsfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
UseTagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH26_pBad_AraC_Para-sfGFP.1TAG
Plasmid#163721PurposeArabinose-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertaraC gfp.1TAG
UseTagsExpressionBacterialMutationgfp.N39TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH27_pBad_AraC_Para-sfGFP.2TAG
Plasmid#163722PurposeArabinose-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertaraC gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH25_pBad_AraC_Para-sfGFP.0TAG
Plasmid#163720PurposeArabinose-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertaraC gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ190b-sfGFP
Plasmid#162447PurposeExpression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentrationDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagsExpressionMammalianMutationtruncation from aa88 to aa249PromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Alpha-Actinin-19
Plasmid#56477PurposeLocalization: Non-muscle Alpha Actinin, Excitation: 485, Emission: 507DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-sACE2v5-sfGFP
Plasmid#145176PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 5DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v4-sfGFP
Plasmid#145175PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.2-sfGFP
Plasmid#149665PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-3xSV40pA-R-td-sfGFP
Plasmid#177150PurposeCAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre / rox ; piggybacTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH807_pJH_LldR_Pcon-sfGFP.2TAG
Plasmid#163738PurposeConstitutive expression of green fluorescent protein encoding two stop codonsDepositorInsertlldr gfp.2TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,Y135TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only