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Plasmid#213555PurposeLentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationN273K mutation in ParkinPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLv-EF1a-Parkin(W403A)-A92mKO2
Plasmid#213558PurposeLentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationW403A mutation in ParkinPromoterEF1aAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.DelHNS IRES Puro
Plasmid#110389Purposeγ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2
Plasmid#124175PurposepiggyBac-based Dox-inducible expression (very weak)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2
Plasmid#124174PurposepiggyBac-based Dox-inducible expression (weak)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorInsertGLS glutaminase (GLS Human)
UseCRISPRTagsExpressionMutationPromoternoneAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
Plasmid#83841PurposeFluorescent probe for G1/S transition and M/G1 transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
mKusabira Orange-N1
Plasmid#55046PurposeEmpty backbone for fusing gene of interest to N-term of mKODepositorTypeEmpty backboneUseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNJP
Plasmid#115494PurposeExpression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cellsDepositorUseTagsiRFP, mCherry, and mKOExpressionMammalianMutationmCherry S227F, mCherry G229R, mKO V211GPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-O
Plasmid#60982PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKODepositorInsertmKO
UseZebrafishTagsExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-mO
Plasmid#60989PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKODepositorInsertmKO
UseZebrafishTagsmembrane tagExpressionMutationPromoterBactin2Available sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-hO
Plasmid#60996PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKODepositorInsertmKO
UseZebrafishTagshistone 2B tagExpressionMutationPromoterBactin2Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-A1:natMX4
Plasmid#134428PurposePCR-based C-terminal A1 tagDepositorInsertA1
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-S6:natMX4
Plasmid#134436PurposePCR-based C-terminal S6 tagDepositorInsertS6
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-A1:hphMX4
Plasmid#134425PurposePCR-based C-terminal A1 tagDepositorInsertA1
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-S6:hphMX4
Plasmid#134433PurposePCR-based C-terminal S6 tagDepositorInsertS6
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-A1:HIS3MX6
Plasmid#134427PurposePCR-based C-terminal A1 tagDepositorInsertA1
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits