-
Plasmid#188510PurposeExpresses FLAG tagged HiFi dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-mCherry
Plasmid#75161PurposeDerived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-EGFP
Plasmid#75159PurposeDerived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-RFP657
Plasmid#75162PurposeDerived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsRFP657ExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-tagBFP
Plasmid#75160PurposeDerived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagstagBFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTHmut
Plasmid#178950PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cellsDepositorInsertAPOBEC1-YTHmut-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AsdCas12a VPR
Plasmid#188512PurposeExpresses FLAG tagged AsdCas12a VPRDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD908APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170810PurposeLentiviral vector to co-express an spsgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HiFi dCas9 KRAB
Plasmid#188500PurposeExpresses FLAG tagged HiFi dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Beclin1
Plasmid#99574PurposeExpressed Cas9 with sgRNA targeting Beclin1DepositorInsertgRNA targeting Beclin1 (BECN1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1-Scr
Plasmid#133042PurposeSiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoExpressionMutationPromoterAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'UAG)
Plasmid#170126PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'UAG)
Plasmid#170128PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'UAG)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgControl
Plasmid#230080PurposeCrispr knock out controlDepositorInsertnon-specific sequence control
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Syn1-mCherry
Plasmid#222967PurposeMake lentivirus to express mCherry in syn1 expressing cellsDepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromotersynapsin IAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170812PurposeLentiviral vector to co-express an sasgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only