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NLS-HA-tdMCP-tdTomato

Plasmid

#183938

Purpose

tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdMCP-tdTomato (cp Synthetic)

Use

Tags

NLS-HA

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

CAGG

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-tDTomato

Plasmid

#191207

Purpose

tdTomato expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Insert

TdTomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pL0M-S-tdTomato-EC18158

Plasmid

#137078

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-tdTomato

Plasmid

#179794

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

tdTomato

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mT2A-tdTomato-FNF

Plasmid

#182340

Purpose

Recombineering cassette plasmid for T2A-tdTomato-FNF

Depositor

Mario Capecchi

Insert

T2A-tdTomato-FNF

Use

Mouse Targeting; Flp / frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-tdTomato-Omp25

Plasmid

#214400

Purpose

Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

Depositor

Liting Duan

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

Insert

iLID-tdTomato-Omp25 (Synj2bp Synthetic, Rat)

Use

Tags

iLID-TdTomato-Omp25

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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p2301y-tdtomato-dCas9_DegronDead_TPLN188

Plasmid

#205409

Purpose

dCas9 guided repressor

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

dCas9_DegronDead_TPLN188

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
AAV-CAG-hChR2_tdTomato

Plasmid

#28015

Purpose

Expresses humanized ChR2 fused to tdTomato for optogenetic activation

Depositor

Karel Svoboda

Article

Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Insert

humanized ChR2-tdTomato

Use

AAV; Adeno associate viral

Tags

tdTomato

Expression

Mutation

Promoter

CAG

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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HES7-NLuc-2A-tdTomato

Plasmid

#130932

Purpose

Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Use

Tags

NanoLuc and tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pTIGER_tdTomato::3xFLAG::dCrk

Plasmid

#131138

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

3XFLAG and tdTomato

Expression

Insect

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mAmetrine-tdTomato-FRET

Plasmid

#58165

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Type

Empty backbone

Use

Tags

mAmetrine and tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-ytdTomato-CaURA3

Plasmid

#118461

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pME tdTomato-NLS

Plasmid

#135205

Purpose

Red fluorescent tag

Depositor

David Tobin

Insert

tdTomato

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:Chronos-tdTomato

Plasmid

#124232

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

Chronos-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pXD042 dTomato KIKO

Plasmid

#192154

Purpose

dTomato KIKO

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

dTomato

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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