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Showing: 241 - 260 of 562 results

  1. BDH2_FL_BioN

    Plasmid
    #195870
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BDH2 (BDH2 Human)
    Use
    Pfbd-bira, baculovirus expression
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195870-plasmid-map-sequence-id-387243

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  4. mycBioID-pBABE-puro

    Plasmid
    #80901
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    BirA(R118G) and myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80901-plasmid-map-sequence-id-158468

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  6. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    35700-plasmid-map-sequence-id-228311

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  9. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

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  10. pENTR_L1-YFP-Turbo-L2

    Plasmid
    #127350
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127350-plasmid-map-sequence-id-246303

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  13. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  14. R4pGWB601_UBQ10p-Turbo-NES-YFP

    Plasmid
    #127366
    Purpose
    Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127366-plasmid-map-sequence-id-246342

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  15. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  16. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Use
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  17. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

    Plasmid
    #127354
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127354-plasmid-map-sequence-id-246338

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  18. pENTR_L1-YFP-Turbo-NES-L2

    Plasmid
    #127349
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127349-plasmid-map-sequence-id-246309

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  19. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid
    #127356
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and mVenus
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127356-plasmid-map-sequence-id-246285

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  20. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid
    #127351
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127351-plasmid-map-sequence-id-246289

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Showing: 241 - 260 of 562 results