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Showing: 601 - 650 of 651 results

  6. pTEV5_ RpMatB_1

    Plasmid
    #78205
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7
    Insert
    matB (His6 C106A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pCCl-MCS_miniCMV_miRFP703

    Plasmid
    #134985
    Purpose
    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector
    Depositor
    Claudio Mussolino
    Article
    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d
    Insert
    miRFP703
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    minCMV
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134985-plasmid-map-sequence-id-265014

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  15. piRFP

    Plasmid
    #31857
    Purpose
    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
    Depositor
    Vladislav Verkhusha
    Article
    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103
    Has Service
    Cloning Grade DNA
    Insert
    iRFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…
    Promoter
    CMV
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    31857-plasmid-map-sequence-id-396432

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  19. pTEV5_ RpMatB_2

    Plasmid
    #78206
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A S170A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A S170A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78206-plasmid-map-sequence-id-146045

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  22. pSW002-Pc-mRuby2

    Plasmid
    #111250
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mRuby2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mRuby2 is codon optimized for expression in P. fl…
    Promoter
    Pc
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111250-plasmid-map-sequence-id-215255

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  29. pTEV5_ RpMatB_3

    Plasmid
    #78207
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A T167A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A T167A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    78207-plasmid-map-sequence-id-152439

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  33. pSW003-Pc-mNeonGreen

    Plasmid
    #205018
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205018-plasmid-map-sequence-id-407997

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  35. pSW002-Pc-mNeonGreen

    Plasmid
    #205016
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    205016-plasmid-map-sequence-id-424184

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  37. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid
    #111254
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA(sp)-mTurquoise2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mTurquoise2 is codon optimized for expression in …
    Promoter
    Pc
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111254-plasmid-map-sequence-id-215265

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  40. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid
    #205020
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorA-mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205020-plasmid-map-sequence-id-407993

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  41. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  48. Peroxisomes-FAST-IRES-iRFP670

    Plasmid
    #216347
    Purpose
    For testing localization of pFAST and iRFP670 proteins to peroxisomes
    Depositor
    Alison Tebo
    Insert
    pFAST_SKL-IRES-iRFP670_PXMP2
    Use
    Tags
    myc for FAST protein only
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216347-plasmid-map-sequence-id-427406

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  49. Mito-FAST-IRES-iRFP670

    Plasmid
    #216346
    Purpose
    For testing localization of pFAST and iRFP670 proteins to mitochondria
    Depositor
    Alison Tebo
    Insert
    Cox8_pFAST-IRES-Tomm20_iRFP670
    Use
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216346-plasmid-map-sequence-id-427397

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  50. ER-FAST-IRES-iRFP670

    Plasmid
    #216344
    Purpose
    For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum
    Depositor
    Alison Tebo
    Insert
    PRL_pFAST-IRES-CANX_iRFP670
    Use
    Tags
    myc for FAST and iRF670 proteins
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216344-plasmid-map-sequence-id-427396

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Showing: 601 - 650 of 651 results