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Plasmid#173443Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y705EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN082
Plasmid#173444Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y706E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN083
Plasmid#173445Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y705E, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN084
Plasmid#173446Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y708EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y708E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y708EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN085
Plasmid#173447Purposeexpress E. coli E69 (O9a:K30) Wzc_K540M with Y713EDepositorInsertE. coli E69 (O9a:K30) Wzc_K540M with Y713E
UseTagshexa-histidine tagExpressionBacterialMutationK540M, Y713EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN072
Plasmid#167953Purposeexpress E. coli E69 (O9a:K30) Wzc with Y705EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y705E
UseTagshexa-histidine tagExpressionBacterialMutationY705EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN073
Plasmid#167954Purposeexpress E. coli E69 (O9a:K30) Wzc with Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y706E
UseTagshexa-histidine tagExpressionBacterialMutationY706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN074
Plasmid#167955Purposeexpress E. coli E69 (O9a:K30) Wzc with Y705E, Y706EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y705E, Y706E
UseTagshexa-histidine tagExpressionBacterialMutationY705E, Y706EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN075
Plasmid#167956Purposeexpress E. coli E69 (O9a:K30) Wzc with Y708EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y708E
UseTagshexa-histidine tagExpressionBacterialMutationY708EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN076
Plasmid#167957Purposeexpress E. coli E69 (O9a:K30) Wzc with Y713EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y713E
UseTagshexa-histidine tagExpressionBacterialMutationY713EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN077
Plasmid#167958Purposeexpress E. coli E69 (O9a:K30) Wzc with Y715EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y715E
UseTagshexa-histidine tagExpressionBacterialMutationY715EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBN078
Plasmid#167959Purposeexpress E. coli E69 (O9a:K30) Wzc with Y717EDepositorInsertE. coli E69 (O9a:K30) Wzc with Y717E
UseTagshexa-histidine tagExpressionBacterialMutationY717EPromoteraraBADAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AP1298‐1
Plasmid#87248Purposegtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)DepositorInsertgtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-FLEX-XRI-FLAG
Plasmid#178057PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.DepositorInsertXRI-FLAG
UseAAVTagsFLAG-MBPExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKate2-B_UTR
Plasmid#68480PurposeLentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKate2-B_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a
Plasmid#80611PurposepBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB
Plasmid#80610PurposepLIB - Library vector (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b
Plasmid#80612PurposepBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAgR_tdTomato
Plasmid#102993PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mTagBFP2-A_UTR
Plasmid#68479PurposeLentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mTagBFP2-A_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "A" added to 3…PromoterCMV and pGKAvailable sinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniSOG
Plasmid#193913PurposeControl plasmid that expresses non-targeted miniSOG in mammalian cellsDepositorInsertminiSOG
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-PtBle
Plasmid#202180PurposeLevel 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.DepositorInsertShBle expression cassette
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-CEN_ARS_HIS
Plasmid#202129PurposeLevel 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.DepositorInsertCEN_ARS_HIS
UseSynthetic BiologyTagsExpressionMutationARS sequence mutated to remove sapI site for dome…PromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK599
Plasmid#110600PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGK:TRL-EFS:iRFP670
Plasmid#68475PurposeLentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670DepositorInsertstTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFS and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
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STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TOPO rtTA3-2A-Cre
Plasmid#68448PurposeTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible geneDepositorInsertrtTA3-2A-Cre
UseGmapTagsExpressionBacterialMutationCodon optimized for expression in micePromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-H4
Plasmid#202178PurposeLevel 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertH4 terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-fcpA
Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-H4
Plasmid#202122PurposeLevel 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertH4 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-P49
Plasmid#202120PurposeLevel 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertP49 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172504PurposeEncodes the GwCas7x3 protein only for bacterial recombinant protein expression for purificationDepositorInsertGwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV2A-T
Plasmid#153022PurposeSingle-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK001
Plasmid#110573PurposeBroad-host-range toolkit (BTK) entry vector, with superfolder GFPDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKO2-C_UTR
Plasmid#68481PurposeLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKO2-C_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "B" added to 3…PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK599s
Plasmid#110601PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits