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Plasmid#143380PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2823
Plasmid#143629PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIKZF1 (IKZF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_iNEUROD1_P2A_GFP_Puro
Plasmid#168803PurposePiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expressionDepositorInsertNEUROD1 (NEUROD1 Synthetic, Human)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-SWAP70
Plasmid#84581PurposeExpresses GFP-tagged SWAP70 in mammalian cellsDepositorInsertSWAP70 (SWAP70 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC7A9_STOP
Plasmid#161411PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC7A9 (SLC7A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP TEM4 FL
Plasmid#58893PurposeExpresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.DepositorInsertTEM4 (ARHGEF17 Human)
UseTagsEGFPExpressionMammalianMutationL722FPromoterCMVAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CSNK2A1
Plasmid#23678DepositorInsertCSNK2A1 (CSNK2A1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP
Plasmid#58472PurposeExpresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoterDepositorInsertUtr230 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CAPNS1_EF-hand_8+EF-hand_5
Plasmid#109995PurposeProtein expression and purification of CAPNS1_EF-hand_8+EF-hand_5DepositorInsertCAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEX-PK-hLC3∆G
Plasmid#61459PurposeExpress pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagyDepositorInsertPK-hLC3∆G (MAP1LC3B Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-WT
Plasmid#134645Purposelysosome-targeted Sac1 catalytic domain wild typeDepositorInsertSac1 catalytic domain (77-520 aa) (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationcontains only amino acids 77-520 (Please see depo…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
UseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFORF1181
Plasmid#143475PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX21 (TBX21 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
UseTagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArgininePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Septin 2 -tdTomato
Plasmid#118735PurposeTo express Septin 2 tagged with tdTomato on C-terminusDepositorInsertSeptin 2 (SEPTIN2 Human)
UseLentiviralTagstdTomatoExpressionMutationPromoterCMVAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…PromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hADARB2-3xFLAG
Plasmid#165470PurposeHuman ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.DepositorInsertAdenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-TGAT
Plasmid#84334PurposeExpresses TGAT tagged with mCherryDepositorInsertTGAT (TRIO Human)
UseTagsmCherryExpressionMammalianMutationWTPromoterCMVAvailable sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-F-PA1
Plasmid#40618DepositorInsertPA1 (PAGR1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only