Showing: 2561 - 2580 of 6451 results
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Plasmid#51881Purposeexpression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 R152Q (tpte2 Frog)
UseXenopus oocyte expressionTagsExpressionMammalianMutationR152Q: near the extracellular side of the fourth …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
1208 p3e-long etv2 3’UTR
Plasmid#49010PurposeZebrafish Long length etv2 3' UTR in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryTagsExpressionMutationLongest 3' UTRPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-HA-shift-IRES-GFP(KaganE21)
Plasmid#52004PurposeExpresses the human MAVS CDS containing an N-terminal HA tag and a frameshift mutation inserting TA at bp number 254 of the MAVS CDS . And a GFP markerDepositorInsertMAVS-Shift
UseRetroviralTagsHAExpressionMammalianMutationA 2 nucleotide insertion was introduced between t…PromoterAvailabilityAcademic Institutions and Nonprofits only -
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p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)
Plasmid#52895PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianMutationPromoterUnknownAvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CDK7 D155A HA (P#812)
Plasmid#14648DepositorInsertCdk7 (CDK7 Human)
UseTagsHAExpressionMammalianMutationD155A: dominant negativePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Smad1 (4SP/AP+AAVA)
Plasmid#14955DepositorInsertSmad1 (4SP/AP+AAVA) (SMAD1 Human)
UseTagsFlagExpressionMammalianMutationFour Serine to Alanine mutations in the linker re…PromoterAvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 1
Plasmid#8477DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationD42APromoterAvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 2
Plasmid#8478DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationS72DPromoterAvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 5
Plasmid#8479DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationG192DPromoterAvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 6
Plasmid#8480DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationT208DPromoterAvailabilityAcademic Institutions and Nonprofits only -
myc GbetaL mutant 7
Plasmid#8481DepositorInsertGbetaL (MLST8 Human)
UseTagsmycExpressionMammalianMutationF320SPromoterAvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG MSCV
Plasmid#8515DepositorInsertHOXA9 (Hoxa9 Mouse)
UseRetroviralTagsFLAG (DYKDDDDK) and IRES-GFPExpressionMammalianMutationmakes GFP from an IRES driving translation from a…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2561 - 2580 of 6451 results