162,052 results

1. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4X PRE TK luc

   Plasmid

   #206159

   Purpose

   Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   4X PRE TK luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. miniCMV-(mNeonGreen)4-tDeg

   Plasmid

   #129402

   Purpose

   (mNeonGreen)4-tDeg fluorogenic protein

   Depositor

   Samie Jaffrey

   Article

   Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

   Insert

   (mNeonGreen)-tDeg
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209661

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   GFAP

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-SRRM2-mCherry

   Plasmid

   #174089

   Purpose

   Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

   Depositor

   Katie Ullman, Wesley Sundquist

   Article

   Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

   Insert

   SRRM2 (SRRM2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Sept. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZT-C13-L1

   Plasmid

   #62196

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Left CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti.PGK.LifeAct-Ruby.W

   Plasmid

   #51009

   Purpose

   Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

   Depositor

   Rusty Lansford

   Insert

   LifeAct-mRFPruby
   

   Use

   Lentiviral

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   human PGK

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRUPATH Triple (bidirectional) GasS

   Plasmid

   #196055

   Purpose

   Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphasS-RLuc8
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUltra-puro-RTTA3

    Plasmid

    #58750

    Purpose

    silence doxycyclin inducible pUltra-dox

    Depositor

    Yildirim Dogan, Kitai Kim

    Insert

    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Ubiquitin C promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0058

    Plasmid

    #143007

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MAF (MAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-CMV-Luc2-IRES-ZsGreen-W

    Plasmid

    #164432

    Purpose

    Lentiviral vector backbone encoding firefly luciferase and ZsGreen

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Inserts

    Luciferase
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGART-GFP

    Plasmid

    #99106

    Purpose

    Expresses human GART-GFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    GART (GART Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A65T, L68V, and A72S mutations in GFP gene; kanR/…

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-tetO-NGN2

    Plasmid

    #197090

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ER-RA

    Plasmid

    #209872

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-GCaMP6-150

    Plasmid

    #86918

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-150
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-tagged NRP1 (human)

    Plasmid

    #213068

    Purpose

    Express myc-NRP1 in mammalian cells

    Depositor

    Yoav Henis

    Insert

    neuropilin 1 (NRP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal myc tag was introduced by a three-step…

    Promoter

    T7

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUCP20T-tdTomato

    Plasmid

    #78469

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-HA-USP18

    Plasmid

    #22572

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP18 (USP18 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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