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pSIN-LexAOx4-Pmin-mCherry

Plasmid

#192633

Purpose

Lentiviral vector for inducible mCherry expression.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Use

Tags

mCherry- AID

Expression

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKC96 pBABE H2B-mCherry

Plasmid

#192695

Purpose

Retroviral transgene expression of human H2B

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

H2B

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-NLSmCherryNLS Clover-HDHB

Plasmid

#190223

Purpose

Fluorescent cell cycle reporter

Depositor

Laura Heiser

Article

Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

Inserts

Human DNA Helicase B (amino acids 994-1087) (HELB Human)
NLS-mCherry-NLS-P2A-Puromycin

Use

Transposon

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Amino Acids 994-1087

Promoter

EF1a and RPBSA

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-Cenexin-S796A

Plasmid

#186434

Purpose

Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

Insert

Cenexin (ODF2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 796 to Alanine

Promoter

CMV IE94

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2-mEGFP

Plasmid

#182859

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Use

Tags

LEXY and mCherry

Expression

Insect

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-50-DExogron-mCherry-R11b

Plasmid

#179904

Purpose

Donor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-23-DExCon-mCherry-R11b

Plasmid

#179898

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mCherry2-(L)-mApple

Plasmid

#182858

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-PGL-1-mHP1alpha

Plasmid

#181894

Purpose

Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mouse, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-nE-mHP1alpha-delCTE

Plasmid

#181891

Purpose

Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-MCP-mCherry

Plasmid

#180275

Purpose

to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

MCP-mCherry

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180477

Purpose

Inducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Inserts

5x QUAS-ZsGreen-P2A
mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Hy_pUbi-p63e mCherry SV40

Plasmid

#176300

Purpose

Expresses mCherry mRNA from the Drosophila Ubi-p63e promoter

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Ubi-p63e

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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