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Plasmid#114141PurposeMammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5'
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Bmf (S74D)
Plasmid#24259DepositorInsertBmf (S87D) (BMF Human)
UseTagsFlagExpressionMammalianMutationchanged Serine 74 to Aspartic AcidPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Bmf (S74A)
Plasmid#24258DepositorInsertBmf (S87A) (BMF Human)
UseTagsFlagExpressionMammalianMutationchanged Serine 74 to AlaninePromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H02
Plasmid#70956PurposeGateway entry cloneDepositorInsertIswi (Iswi Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATF4 19: (SL)RLuc.5’ATF4.GFP
Plasmid#21865DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStem Loop upstream of ATF4PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrc-T7Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (T56D)
Plasmid#24237DepositorInsertBimL (T56D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged threonine 56 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
720-2µ-maxHIS3
Plasmid#40612DepositorInsertHIS3 (HIS3 Synthetic, Budding Yeast)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGex2t SadBs
Plasmid#66882PurposeBacterial expression vector encoding N-terminally GST-tagged short isoform of human SadBDepositorInsertSadB (BRSK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S58D)
Plasmid#24239DepositorInsertBimL (S58D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 58 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV T274V KSR1 IRES GFP
Plasmid#25977DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged threonine 274 to valinePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV S392A KSR1 IRES GFP
Plasmid#25978DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 392 to alaninePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJW3.42
Plasmid#62866PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusionDepositorInsertPPTR-1/pLexA-N bait
UseTwo-hybrid bait vectorTagsLexAExpressionYeastMutationPromoterAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Shp2 SH2s
Plasmid#67575PurposeExpresses GST-tagged Shp2 SH2 domains in bacteriaDepositorInsertSrc homology 2 domain-containing phosphatase 2 SH2s
UseTagsGSTExpressionBacterialMutationDeleted amino acids 219-597PromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psbA2-PHLS (b)
Plasmid#52307PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpsbA2Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis (1-440)
Plasmid#60391PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-PHLS (d)
Plasmid#52309PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S44D)
Plasmid#24235DepositorInsertBimL (S44D) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged serine 44 to aspartic acidPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE
Plasmid#113536PurposeRetroviral vector designed to knock down MePCE.DepositorInsertshMePCE (MEPCE Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (S44A)
Plasmid#24234DepositorInsertBimL (S44A) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationChanged Serine 44 to AlaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only