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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Y921F EphA2 pcDNA3

Plasmid

#102736

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y921F (TAC > TTC)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr4

Plasmid

#108105

Purpose

Retroviral vector for expression of mouse Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45c IRES GFP

Plasmid

#17882

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45c (Dpf3 Mouse)

Use

Tags

FLAG and IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
mEos2-Beta-Catenin-20

Plasmid

#57350

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Beta-Catenin (Ctnnb1 Mouse)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pLEX307-mFzd8

Plasmid

#102870

Purpose

Expresses mouse Fzd8 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 8 (Fzd8 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T to C substitution at nucleotide 1134, C to T su…

Promoter

EF1A

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pOZ-Pax3-FH-C-puro

Plasmid

#32517

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax3 (Pax3 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pLV Tor1a-V5

Plasmid

#175135

Purpose

Lentiviral expression of Tor1a-Myc

Depositor

Larry Gerace

Insert

Tor1a (Tor1a Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-7b

Plasmid

#18026

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-7b (Wnt7b Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pMKO.1-shCcnb1

Plasmid

#162793

Purpose

Ccnb1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb1 shRNA (Ccnb1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PECAM-H2B-GFP-Hygro

Plasmid

#14684

Depositor

Victoria Bautch

Article

Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Use

Tags

H2B-GFP and PGK-Hygro

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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Bin1 N-BAR domain

Plasmid

#168280

Purpose

Express N-BAR domain of Bin1 (aa 1-267) in pEGFP-N1 vector

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-267

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-NINJ1

Plasmid

#201179

Purpose

Expression of NINJ1 gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Galpha-i2

Plasmid

#15929

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-i2 (Gnai2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
jk96

Plasmid

#120177

Purpose

CAG:mcherry:P2A:HA_NLGN

Depositor

Anthony Zador

Insert

CAG:mCherry:P2A:HA_NLGN

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Satb1

Plasmid

#74357

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Satb1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

special AT-rich sequence binding protein 1 (Satb1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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CamKII alpha Venus

Plasmid

#29427

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CamKII alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
MKK7

Plasmid

#29576

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK7 (Map2k7 Mouse)

Use

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 MKK7b1(K149A)

Plasmid

#14674

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(K149A) (Map2k7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Lys-149Ala

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H-2Kb

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pSuper-Rem2-2

Plasmid

#51595

Purpose

shRNA 2 against rodent Rem2

Depositor

Suzanne Paradis

Article

Moore et al PLoS One. 2013 Aug 26;8(8):e74751. doi:

Insert

Rem2 (Rem2, Rem2 Mouse, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

U6

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pSuper-Rem2-1

Plasmid

#51584

Purpose

shRNA 1 against rodent Rem2

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2, Rem2 Mouse, Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

U6

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
attB-mCh-Dnmt3b6-Poly(A)-NeoR

Plasmid

#65554

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 6

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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mPlum-MyosinIIA-C-18

Plasmid

#55989

Purpose

Localization: Cytoskeleton, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

MyosinIIA (Myh9 Mouse)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
mCHOP10(dLZ).pCDNA1

Plasmid

#21900

Depositor

David Ron

Article

Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

Insert

CHOP10 (Ddit3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The coding region of the murine CHOP-10 cDNA was …

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
CAPZB2-TwinStrepII

Plasmid

#83194

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Insert

CAPZB2 (Capzb Mouse)

Use

Tags

Twin Strep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ncam1_1.18.1-AP-His

Plasmid

#71962

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.18.1 (Ncam1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-SUR2A [N319A/14R]

Plasmid

#177530

Purpose

Mammalian Expression Plasmid of anti-SUR2A (Mouse). Derived from hybridoma N319A/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SUR2A (Mus musculus) recombinant mouse monoclonal antibody (Abcc9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5/FRT/DEST-3xHA/mGli2 P1-6A

Plasmid

#51251

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848,923,956 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-MK3

Plasmid

#30188

Depositor

Bruce Tempel

Insert

Kcna3 (Kcna3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
p229_LTJ_2kbCD90.1Template

Plasmid

#82668

Purpose

2kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKS Notch4

Plasmid

#21325

Depositor

Jan Kitajewski

Article

Uyttendaele et al Development. 1996 Jul . 122(7):2251-9.

Insert

Notch4 (Notch4 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
GFP-nArgBP2ΔNSE

Plasmid

#74515

Purpose

Express GFP-nArgBP2ΔNSE fusion protein in mammalian cells. nArgBP2ΔNSE is the mutant without the neuronal specific exon of nArgBP2.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2delNSE (Sorbs2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFL-mPML-3KRmut-iB

Plasmid

#140287

Purpose

Expression of mouse PML KR mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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