FLAG TBC1D13
(Plasmid #87953)

• Purpose: Expresses flag tagged mouse TBC1D13
• Depositing Lab: David James
• Publication: Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 10.1111/j.1600-0854.2012.01397.x. Epub 2012 Jul 29.

Backbone

• Vector backbone: p3xFLAG CMV10
• Backbone size w/o insert (bp): 6400
• Total vector size (bp): 7600
• Modifications to backbone: This vector has undergone site-directed mutagenesis to bring the TBC1D13 insert into frame with the tag. The EcoRI site was modified by inserting a base in this sequence and hence can no longer be digest with this enzyme
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TBC1D13
• Alt name: TBC1 domain family member 13
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Entrez Gene: Tbc1d13 (a.k.a. 2600014A06Rik, BC025586)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV_F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: p3xFLAG-CMV19 from Sigma. TBC1D13 was originally cloned by the depositing scientist.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry