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Plasmid#193617PurposeExpresses MSLNDepositorInsertMSLN (MSLN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorInsertSynaptophysin-Lime (Syp Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClathrin-miRFP670nano3
Plasmid#184667PurposeClathrin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-clathrin light chain
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSE401
Plasmid#62201PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_ChR2_mCherry
Plasmid#213941PurposeAAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherryDepositorInsertChannelrhodopsin 2-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
dimericTomato-2xrGBD
Plasmid#176098PurposeVisualization of active RhoADepositorInsertrhotekin (Rtkn Mouse)
UseLentiviralTagsExpressionMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-RCaMP1h
Plasmid#105013Purposemitochondrial calcium sensorDepositorInsertRCaMP1h
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1112
Plasmid#143927PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRCOR3 (RCOR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Pam-FL
Plasmid#42570DepositorInserthuman MYCBP2 (MYCBP2 Human)
UseTagsMycExpressionMammalianMutationPam-FL clone lacks alternatively spliced exons 52…PromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBUE411
Plasmid#62200PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3M14nes
Plasmid#157855PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertUseTagsExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395APromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-As-crRNA
Plasmid#78956PurposeCloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cre
Plasmid#34564DepositorInsertCre
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP-h-PKD2
Plasmid#83451PurposeGFP-tagged PC2 for Eukaryotic expression (883)DepositorInsertPolycystin-2 (PKD2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-527
Plasmid#11518PurposeGateway destination vector for bacterial expression; His tagDepositorTypeEmpty backboneUseGateway destination vectorTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-WT
Plasmid#64060PurposeEGFP fused at the N-terminus of tubulin; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-Ubiquitin
Plasmid#74218PurposeLentiviral expression of HA tagged UbiquitinDepositorInsertubiquitin (UBB Human)
UseLentiviralTagsHAExpressionMammalianMutationSingle ubiquitin coding sequence (full length UBB…PromoterCMVAvailable sinceMarch 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K33
Plasmid#17607PurposeMammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK33 only, other lysines mutated to arginines; P19…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKT0127
Plasmid#8728DepositorInsertyEGFP
UseTagsCodon optimized linkerExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.P301L-Tau
Plasmid#140425PurposeAAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTauDepositorInserteGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
UseAAVTagseGFPExpressionMutationchanged Proline 301 to LeucinePromoterCBAAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP
Plasmid#10676DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(M1-40)
Plasmid#71876PurposeExpresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cellsDepositorInsertAbeta(M1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)
Plasmid#191391PurposeDonor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locusDepositorInserthCdt1(30-120)
UseCRISPR and Synthetic BiologyTagsmScarlet-IExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato
Plasmid#166600PurposeEncodes Cre-dependent ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVTagsExpressionMutationH134RPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only