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Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterMaize ubiquitin 1 (ZmUbi1)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo largeTcDNA
Plasmid#1780PurposeRetroviral expression of Large T Antigen. Used to create immortalized cell lines.DepositorInsertlarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-HO-1
Plasmid#80345PurposeExpresses smURFP and Heme Oxygenase-1 in mammalian cells.DepositorInsertssmURFP
HO-1
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MP2
Plasmid#36097Purpose3rd generation lentiviral plasmidDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1
Plasmid#47888PurposeExpresses myc tagged Miro1DepositorInsertMiro1 (RHOT1 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP
Plasmid#30130DepositorInsertEnhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Plasmid#44362PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAV; Adeno associated viral vectorTagsmCherryExpressionMutationPromoterhuman Synapsin 1Available sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-CMV
Plasmid#16403PurposeShuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-HRP-KDEL
Plasmid#85582PurposeExpresses HRP-KDEL in mammalian cellsDepositorInsertHRP-KDEL
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorInsertCullin 4B (CUL4B Human)
UseTags3xmycExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only