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Showing: 2541 - 2560 of 2721 results

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-AID

Plasmid

#193647

Purpose

Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hAID, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-BE

Plasmid

#193648

Purpose

Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-rAPOBEC1, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-CDA1

Plasmid

#193649

Purpose

Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-PmCDA1, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-eAID

Plasmid

#193650

Purpose

Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-eAID, EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr3

Plasmid

#193660

Purpose

Expression of tandem pre-sgRNA array hcr3 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr5

Plasmid

#193661

Purpose

Expression of tandem pre-sgRNA array hcr5 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-pcr4

Plasmid

#193662

Purpose

Expression of tandem pre-sgRNA array pcr4 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-GFAP R416WT scFv [N206B/9] with sortase tag

Plasmid

#204420

Purpose

Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Calbindin scFv [L109/57] with sortase tag

Plasmid

#204421

Purpose

Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting Calbindin (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-PSD-95 scFv [K28/43] with sortase tag

Plasmid

#204422

Purpose

Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting PSD-95 (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Parvalbumin scFv [L114/81] with sortase tag

Plasmid

#204423

Purpose

Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-VGluT1 scFv [N28/9] with sortase tag

Plasmid

#204424

Purpose

Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting VGluT1 (Rattus norvegicus)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Kv1.2 scFv [K14/16] with sortase tag

Plasmid

#204425

Purpose

Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

Plasmid

#204426

Purpose

Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

Plasmid

#160631

Purpose

Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.

Depositor

Diego Orzaez

Insert

gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

U626

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

Plasmid

#65417

Purpose

AAV packaging vector for hSyn driven KOR DREADD expression.

Depositor

Bryan Roth

Article

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Has Service

AAV8

Insert

hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Expression

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9P35S-H

Plasmid

#66189

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9P35S-B

Plasmid

#66190

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Availability

Academic Institutions and Nonprofits only

Enlarge