Addgene

We narrowed to 17,033 results for: gateway

Showing: 2521 - 2540 of 17033 results

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IO001: pMVP (R4-R3) eGFP

Plasmid

#121730

Purpose

pMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116A

Plasmid

#123081

Purpose

Gateway entry clone encoding human GABARAP G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 116 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-HygTK-L2

Plasmid

#141006

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HygTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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IO201: pMVP (R4-R3) mCherry

Plasmid

#121732

Purpose

pMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-TAZ CA

Plasmid

#79504

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WWTR1 (WWTR1 Human)

Use

Multisite gateway entry clone

Tags

3xFLAG

Expression

Mutation

S89A

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-CAG-R3

Plasmid

#141036

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

CAG promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G06

Plasmid

#67140

Purpose

Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G09

Plasmid

#67143

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

Plasmid

#141025

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-Neo-L2

Plasmid

#141007

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG Luciferase

Plasmid

#98391

Purpose

Gateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

Luciferase

Use

Entry vector for gateway cloning

Tags

3xFLAG

Expression

Mutation

Promoter

TRE tight and TRE tight promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-PuroTK-L2

Plasmid

#141005

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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