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Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-LMNB1
Plasmid#207770PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.DepositorArticleInsertsgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2h
Plasmid#208721PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF1
Plasmid#200384PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0mut
Plasmid#208718PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SaCas9v3-Puro
Plasmid#178813PurposeSaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12aUltra_5XNLS
Plasmid#218775PurposeHis-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLSDepositorInsert5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
UseCRISPRTags6XHis (C terminal), HA, Nucleoplasmin, SV40, and …ExpressionBacterialMutationNucleotides mutations were made to encode for arg…PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-SUN1
Plasmid#213515PurposeExpresses human eGFP-SUN1 in mammalian cellsDepositorInsertSUN1 (SUN1 Human)
UseTagsExpressionMammalianMutationSUN1 has a silent mutation on its gene (nucleotid…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_r5-HT1.0
Plasmid#208713PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cellsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-OPTN (2-119)
Plasmid#208867PurposeStably express GFP-tagged OPTN (2-119aa) for CID induced mitophagyDepositorInsertOPTN (OPTN Human)
UseTagsFKBP-GFPExpressionMammalianMutationaa 2-119 onlyPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2h
Plasmid#208709PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF2
Plasmid#200385PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF0
Plasmid#200383PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0
Plasmid#208711PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G
Plasmid#216111PurposeExpresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.DepositorInsertoROS-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only