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pAAV-nEF-ConVERGD-eGFP-WPRE

Plasmid

#218747

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

nEF

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-ConVERGD-eGFP-W3SL

Plasmid

#218746

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

nEF

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-2A-dTomato

Plasmid

#51902

Purpose

pre-2Ato

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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HPRT

Plasmid

#22884

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)

Use

Cre/Lox; Homologous recombination

Tags

Expression

Mutation

PGK, NeoR, and BGH polyA are between the two geno…

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 FLEX NAPA-N SV40

Plasmid

#92283

Purpose

FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

AAV

Tags

Expression

Mutation

Promoter

hSynapsin1

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDD283

Plasmid

#66824

Purpose

YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

YPET-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized YPET

Expression

Worm

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Scr shRNA

Plasmid

#59299

Purpose

non-targeting shRNA

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Scr shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-ConVERGD-eGFP-WPRE

Plasmid

#218745

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-eGFP-WPRE

Plasmid

#218743

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-ReaChR-P2A-dTomato

Plasmid

#114368

Purpose

expresses a Cre-dependent red light-shifted channelrhodopsin variant

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

ReaChR-P2A-dTomato

Use

AAV

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-RG

Plasmid

#48333

Purpose

Cre-dependant (flex) rabies glycoprotein

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

Rabies Glycoprotein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer1

Plasmid

#14763

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLIP-puro-dsRed-GFP-miRNA

Plasmid

#32704

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

emGFP-miRNA

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-LSL-mCherry-shLuci

Plasmid

#178590

Purpose

Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Luciferase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-W3SL

Plasmid

#218744

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKHR5

Plasmid

#74593

Purpose

an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mutation

Promoter

alpha-crystallin

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxOFF-pre-mGRASP-mCerulean

Plasmid

#51900

Purpose

JxOFF-pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 FLAG

Plasmid

#17521

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pSicoR human Drosha1

Plasmid

#14766

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 Apr 1. ():.

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

Plasmid

#50022

Purpose

expresses Membrane tagged G-CaMP3 in cells expressing Cre

Depositor

David Anderson

Article

Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

Insert

G-CaMP3

Use

AAV

Tags

MARCKS sequence (MGCCFSKT)

Expression

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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