Addgene

Showing: 2521 - 2540 of 30455 results

Results per page:

Sort results by:

PyronicSF-mRuby2/pBI-CMV1

Plasmid

#124830

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

Inserts

PyronicSF
mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-AID-Ns3h-UGI

Plasmid

#221219

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/myc/Nuc-hOGG1

Plasmid

#18709

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 . 6():235.

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Expression

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-GEM-GECO1

Plasmid

#32461

Purpose

Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

GEM-GECO

Use

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Sport6-CD81-pHluorin

Plasmid

#130903

Purpose

Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD81-pHluorin (CD81 Human, aequorea victoria)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Leu-49 and Gly-50 in ex…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Bsd-CMV-MDM4

Plasmid

#202669

Purpose

MDM4 overexpression vector with blasticidin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 regulator of p53 (MDM4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

GENIE Project, Douglas Kim

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpRYc-ABE8e-EGFP

Plasmid

#208340

Purpose

Expresses SpRYc-ABE8e in mammalian cells

Depositor

Pranam Chatterjee

Article

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

ecTadA(8e)-nSpRYc

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-tomm20-ECFP-RspANFAST

Plasmid

#216348

Purpose

targets ECFP-RspANFAST to mitochondrial membrane

Depositor

Alison Tebo

Insert

tomm20-ECFP-RspANFAST

Use

Tags

tomm20

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CMV GCGR-V5-sTurboID (N)

Plasmid

#153009

Purpose

Expresses split-TuboID N-terminal fragment fused to GCGR

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

GCGR-sTurboID (N) (L73)

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLAG-CMV4-ZIKV-NS2A

Plasmid

#178842

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-TRE3G-N-tdT-CMV-P

Plasmid

#192887

Purpose

Tet-inducible Nanoluc-tdTomato expression

Depositor

Kazuhiro Oka

Insert

Nanoluc-P2A-tdTomato

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-VSV-G(P127D)-Myc

Plasmid

#80055

Purpose

Expression of a Myc tagged VSV-G protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VSV-G-Myc(P127D)

Use

Tags

Myc

Expression

Mammalian

Mutation

P127D

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge