pFLAG-CMV4-ZIKV-NS2A
(Plasmid
#178842)
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PurposeMammalian expression of ZIKV ORF
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 178842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFLAG-CMV-4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZIKV-NS2A
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SpeciesZika Virus
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Sequence from PRVABC59 genome (GenBank Sequence Accession:KX377337)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV4-ZIKV-NS2A was a gift from Judy Lieberman (Addgene plasmid # 178842 ; http://n2t.net/addgene:178842 ; RRID:Addgene_178842) -
For your References section:
Decidual NK cells kill Zika virus-infected trophoblasts. Sen Santara S, Crespo AC, Mulik S, Ovies C, Boulenouar S, Strominger JL, Lieberman J. Proc Natl Acad Sci U S A. 2021 Nov 23;118(47):e2115410118. doi: 10.1073/pnas.2115410118. 10.1073/pnas.2115410118 PubMed 34785597