Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 2521 - 2540 of 16002 results

  1. pGBW-m4134209

    Plasmid
    #152331
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    152331-plasmid-map-sequence-id-295343

    Enlarge

  3. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs140428747
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

    Enlarge

  9. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Article
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    pHluorin-mKate2 fusion
    Use
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

    Enlarge

  13. p5A0-T

    Plasmid
    #92016
    Purpose
    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    J23102 Promoter (from Anderson promoter library) …
    Availability
    Academic Institutions and Nonprofits only
    92016-plasmid-map-sequence-id-201851

    Enlarge

  14. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

    Enlarge

  15. pPI228

    Plasmid
    #113213
    Purpose
    safe haven knock-in plasmid for the act5 locus, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Safe haven knock-in plasmid for the act5 locus, n…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113213-plasmid-map-sequence-id-217736

    Enlarge

  16. pPI229

    Plasmid
    #113214
    Purpose
    safe haven knock-in plasmid for the act5 locus, C-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Safe haven knock-in plasmid for the act5 locus, c…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113214-plasmid-map-sequence-id-217752

    Enlarge

  18. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

    Plasmid
    #70231
    Purpose
    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope
    Depositor
    Steven Henikoff
    Article
    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.
    Insert
    3xFLAG tag and aa 83-231 of Mnase
    Use
    Yeast genomic targeting
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    70231-plasmid-map-sequence-id-129162

    Enlarge

Showing: 2521 - 2540 of 16002 results