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Plasmid#48157PurposeTransgenically expresses firefly luciferase protein in T. cruziDepositorInsertluciferase
UseLuciferase; T. cruzi expressionTagsExpressionMutationPromoterT7Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-GBP
Plasmid#67672PurposeMultisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)DepositorInsertanti-GFP nanobody
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM41-EcLonA
Plasmid#38330DepositorInsertLon protease fragment (lon Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationPolyphosphate binding fragment of Lon protease. …PromoterT7Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pJCC_058 FnCas12a D917A
Plasmid#171667PurposeFor bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tagDepositorInsertFnCas12a D917A
UseTags6X His and sortase A sorting motifExpressionBacterialMutationchanged aspartate 917 to alaninePromoterAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
KP201: pMVP (L5-L4) eGFP-P2A
Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
459 pME-etv2
Plasmid#49002Purposefull length coding sequence for zebrafish etv2 gene in gateway middle entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway middle entryTagsExpressionMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
KP301: pMVP (L5-L4) mCherry-P2A
Plasmid#121710PurposepMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.DepositorInsertmCherry-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0108
Plasmid#177041PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoterDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0817
Plasmid#177039PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoterDepositorInserttruncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-C
Plasmid#171726PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-C
Plasmid#171734PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-N
Plasmid#171735PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-N
Plasmid#171727PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-N
Plasmid#171731PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-C
Plasmid#171730PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TAZ CA
Plasmid#79504PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDSDepositorInsertWWTR1 (WWTR1 Human)
UseMultisite gateway entry cloneTags3xFLAGExpressionMutationS89APromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 NSP8
Plasmid#158359PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP8 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-SARS-CoV-2 NSP10
Plasmid#158361PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 NSP10 (ORF1ab )
UseTagsMAC-tagExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
R10-3 /pQE-30
Plasmid#79859PurposeThe first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.DepositorInsertR10-3
UseTagsHisTagExpressionBacterialMutationAequorea victoria green fluorescent protein (avGF…PromoterT5 promoter / lac operatorAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only