Addgene

Showing: 2501 - 2520 of 5448 results

Results per page:

Sort results by:

NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FLAG-EglN2 H358A-pBabe-puro

Plasmid

#22705

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated cDNA: point mutation in codon 358 changi…

Promoter

Available since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV40-EF1a-hBCL10-SO

Plasmid

#75256

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs, EF1a

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hNFATc1/bC - AVITEV

Plasmid

#74059

Purpose

retroviral expression plasmid for human NFATc1/bC with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0 mCherry_K-RasC14

Plasmid

#101286

Purpose

Lentiviral expression of K-Ras C14

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

K-Ras (KRAS Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

C14

Promoter

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75238

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-BimEL(Noxa)-pEGFP-C1

Plasmid

#166761

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Noxa…

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-BimEL(Bik)-pEGFP-C1

Plasmid

#166760

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bik

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Bik BH3) (Bcl2l11, BIK Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bik …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-Puma-4E-pEGFP-C1

Plasmid

#166746

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Puma-4E (BBC3 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-tBid-4E-pEGFP-C1

Plasmid

#166743

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

tBid-4E (Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-BimEL(Bid)-pEGFP-C1

Plasmid

#166759

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bid

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Bid) (Bcl2l11, Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of mouse Bid …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV40-EF1a-hBCL10(1-114)-SO

Plasmid

#75257

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

AS 1-116 (CARD-domain)

Promoter

pMSCV-LTRs, EF1a

Available since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-Keratin-17

Plasmid

#56164

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Keratin (KRT18 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Pet28a_Human_H4

Plasmid

#42633

Depositor

Joe Landry

Insert

Human H4 (H4C1 Human)

Use

Tags

Untagged

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
RGS16

Plasmid

#39140

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RGS16 (RGS16 Human)

Use

Tags

His Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge