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Showing: 5001 - 5040 of 148587 results

  5. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  6. pMinDis.X513-iNicSnFR3a-(CC93)-PM

    Plasmid
    #125122
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(CC93)-PM
    Use
    Tags
    Ig-K-Chain and membrane anchoring sequence-PDGFR
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    T7
    Available since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125122-plasmid-map-sequence-id-245433

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  19. pIVTR(T7A)-SOX9

    Plasmid
    #172302
    Purpose
    In vitro transcription of SOX9
    Depositor
    Patrik Verstreken
    Insert
    SOX9 (SOX9 Human)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Jan. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    172302-plasmid-map-sequence-id-351374

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  20. mApple-CENPB-N-22

    Plasmid
    #54877
    Purpose
    Localization: Nucleus/Centromeres, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    CENPB (CENPB Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    aa 1-169 of CENPB (DNA Binding Domain)
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  28. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid
    #221081
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Article
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Insert
    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221081-plasmid-map-sequence-id-438468

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  32. pAG426-GAL-TAF15

    Plasmid
    #181713
    Purpose
    Galactose inducible expression of TAF15
    Depositor
    Alejandro Chavez
    Insert
    TAF15 (TAF15 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    181713-plasmid-map-sequence-id-436302

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  35. pAG416-GAL-hnRNPA1

    Plasmid
    #181710
    Purpose
    Galactose inducible expression of hnRNPA1
    Depositor
    Alejandro Chavez
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181710-plasmid-map-sequence-id-430953

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  36. GBH-R-cmlc2GFP.1

    Plasmid
    #188004
    Purpose
    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish
    Depositor
    Karl Clark
    Insert
    Targeted mRFP protein trap; secondary marker cmlc2 GFP
    Use
    Targetable insertional mutagen and reporter system
    Tags
    Expression
    Mutation
    Promoter
    Endogenuous Promoter; cmlc2 promotor
    Available since
    March 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    188004-plasmid-map-sequence-id-381042

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  37. pREMI-ZA

    Plasmid
    #183736
    Purpose
    Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii
    Depositor
    Jun Ishii
    Article
    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1
    Insert
    Sh ble
    Use
    Random insertional gene deletion in komagataella …
    Tags
    Expression
    Mutation
    Promoter
    TEF1 promoter
    Available since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    183736-plasmid-map-sequence-id-372719

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  38. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  39. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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Showing: 5001 - 5040 of 148587 results