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Showing: 6201 - 6250 of 14286 results

  12. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid
    #52907
    Purpose
    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    Zipp.-L-IFP-F[1]+Plum
    Use
    Retroviral
    Tags
    L-IFP-F[1]
    Expression
    Mammalian
    Mutation
    Promoter
    Unknown
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid
    #172317
    Purpose
    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    172317-plasmid-map-sequence-id-338787

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  14. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid
    #154779
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    LaG17-SynNotch
    Use
    Tags
    LaG17
    Expression
    Mammalian
    Mutation
    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154779-plasmid-map-sequence-id-310404

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  19. pX330_CALR sgRNA / hSpCas9

    Plasmid
    #172838
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172838-plasmid-map-sequence-id-340912

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  23. pMT3-RhoGC

    Plasmid
    #100031
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC
    Use
    Tags
    1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)
    Expression
    Mammalian
    Mutation
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    100031-plasmid-map-sequence-id-192905

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  28. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  32. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154770
    Purpose
    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, G1 PylT with A41AA and C5…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154770-plasmid-map-sequence-id-309867

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  34. pIK137

    Plasmid
    #65632
    Purpose
    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65632-plasmid-map-sequence-id-111543

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  42. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  43. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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Showing: 6201 - 6250 of 14286 results