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Addgene

M72-IRES-tauRFP2-ACNF TV
(Plasmid #15512)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15512 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS (?)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    M72-IRES-tauRFP2-ACNF TV
  • Alt name
    olfactory receptor M72
  • Alt name
    odorant receptor M72
  • Alt name
    Olfr160
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    14846
  • Mutation
    Targeting vector to insert IRES-tauRFP2-ACNF immediately 3' of the coding region of the olfactory receptor gene M72/Olfr160. The coding region of M72/Olfr160 is not affected.
  • GenBank ID
    BC130240
  • Entrez Gene
    Olfr160 (a.k.a. M72, MOR171-3, Olfr7b)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mouse strain that was derived from this plasmid is available from The Jackson Laboratory: http://jaxmice.jax.org/strain/006687.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M72-IRES-tauRFP2-ACNF TV was a gift from Peter Mombaerts (Addgene plasmid # 15512 ; http://n2t.net/addgene:15512 ; RRID:Addgene_15512)
  • For your References section:

    Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor. Feinstein P, Bozza T, Rodriguez I, Vassalli A, Mombaerts P. Cell. 2004 Jun 11. 117(6):833-46. 10.1016/j.cell.2004.05.013 PubMed 15186782