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Showing: 2481 - 2500 of 3838 results

  2. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  3. pcDNA3-mNeptune2-N

    Plasmid
    #41645
    Purpose
    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    mNeptune2-Dronpa145N
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K145N on Dronpa (allows control of photochromic F…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41645-plasmid-map-sequence-id-296256

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  8. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  11. hCas9

    Plasmid
    #41815
    Purpose
    Expresses human codon optimized Cas9 nuclease for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41815-plasmid-map-sequence-id-106044

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  12. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    Cas9_D10A
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

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  13. gRNA_AAVS1-T1

    Plasmid
    #41817
    Purpose
    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    gRNA_AAVS1-T1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41817-plasmid-map-sequence-id-224317

    Enlarge

  14. gRNA_AAVS1-T2

    Plasmid
    #41818
    Purpose
    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    gRNA_AAVS1-T2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41818-plasmid-map-sequence-id-287407

    Enlarge

  15. gRNA_GFP-T1

    Plasmid
    #41819
    Purpose
    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    gRNA_GFP-T1
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41819-plasmid-map-sequence-id-116036

    Enlarge

  16. gRNA_GFP-T2

    Plasmid
    #41820
    Purpose
    Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Insert
    gRNA_GFP-T2
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41820-plasmid-map-sequence-id-116301

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  17. gRNA_Cloning Vector

    Plasmid
    #41824
    Purpose
    An empty gRNA expression vector, used to create a gRNA to a specific sequence.
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    AflII site for Gibson Assembly
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    41824-plasmid-map-sequence-id-269723

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Showing: 2481 - 2500 of 3838 results