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Plasmid#63204PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
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pAV10.HO3.loxP
Plasmid#63205PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO6
Plasmid#63211PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr (RB3578)
Plasmid#69099PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3prDepositorInsertsyZ3EV cassette
Z3 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
AausFP1-T2A-mCherry pdrf1-GW
Plasmid#168071PurposeProduces equimolar expression of AausFP1 and mCherry for use in yeastDepositorInsertAausFP1-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ystagRFP-T2A-mTq2 pdrf1-GW
Plasmid#168072PurposeProduces equimolar expression of ystagRFP and mCherry for use in yeastDepositorInsertystagRFP-T2A-mTq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mAvicFP1-T2A-mCherry pdrf1-GW
Plasmid#168073PurposeProduces equimolar expression of mAvicFP1 and mCherry for use in yeastDepositorInsertmAvicFP1-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mGreenLantern-T2A-mCherry pDRF1-GW
Plasmid#168060PurposeProduces equimolar expression of mGreenLantern and mCherry for use in yeastDepositorInsertmGreenLantern-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-TLG1
Plasmid#105261PurposePlasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB8644
Plasmid#126919PurposePlasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYBDepositorInsertXdCrtI and HMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8787
Plasmid#126921PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1DepositorInsertHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8748
Plasmid#126918PurposePlasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtEDepositorInsertXdCrtE
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT2-HRP
Plasmid#44021DepositorInsertHRP
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-G418-C4
Plasmid#160342PurposeHigh-copy expression vector for K. marxianus, G418 selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHES835
Plasmid#87946PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HPH
Plasmid#160014PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1
Plasmid#41595DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a LEU2
Plasmid#61923PurposeExpression of K. lactis LEU2 cloned into pFA6aDepositorInsertLEU2
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZalphaB-SapL3
Plasmid#78171PurposeExpression of Saporin L3 in pichiaDepositorInsertSaporin L3
UseTagsalpha factorExpressionYeastMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only