-
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL24_Cas9-His Δcys E1026C
Plasmid#206285PurposeBacterial expression of Cas9-His Δcys E1026C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1026CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL27_Cas9-His Δcys E532C/E945C
Plasmid#206288PurposeBacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574S, E945CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL26_Cas9-His Δcys M1C/E532C
Plasmid#206287PurposeBacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL22_Cas9-His Δcys E532C
Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE
Plasmid#201531PurposeExpressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE
UseSynthetic BiologyTagsExpressionMutationPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3
Plasmid#201532PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3
UseSynthetic BiologyTagsExpressionMutationPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-GFP-9S [rpoA]
Plasmid#176221PurposeExpression of GFP-9S [rpoA]DepositorInsertGFP-9S [rpoA]
UseSynthetic BiologyTagsHis-GFPExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1046E=dgRNAs[traB,bTub]
Plasmid#149425PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.DepositorInsertU6.3-gRNAs[TraB, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterU6.3Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1333-C
Plasmid#174561Purposemitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hNKD2
Plasmid#174329Purposeencodes human NKD2 cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1397-C
Plasmid#174563Purposemitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vectorDepositorInsertDdCBE G1397-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1397-N
Plasmid#174562Purposemitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mt-DdCBE G1333-N
Plasmid#174560Purposemitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1397-C
Plasmid#174559PurposeChloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vectorDepositorInsertDdCBE G1397-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1397-N
Plasmid#174558PurposeChloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vectorDepositorInsertDdCBE G1397-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only