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Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDest_U6_A2_CG2_T1_gRNA
Plasmid#199387PurposepDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNADepositorInsertnrg1 type I sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T2_gRNA
Plasmid#199388PurposepDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNADepositorInsertnrg1 type II sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-AID
Plasmid#196242PurposePlasmid expressing the nCas9-AID fusionDepositorInsertnickase Cas9 fused to AID
UseCRISPRTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest_U6_A2_CG2_T3_gRNA
Plasmid#199389PurposepDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNADepositorInsertnrg1 type III sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK122
Plasmid#200146PurposeType 3b part for Modular Cloning in YeastDepositorInsertsfGFP1-10
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_62 [sfYFP]
Plasmid#198974PurposeLevel 0 partDepositorInsertsfYFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_35 [aadA]
Plasmid#198948PurposeLevel 0 partDepositorInsertaadA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_64 [mScarlet]
Plasmid#198975PurposeLevel 0 partDepositorInsertmScarlet-I
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_57 [PtetL]
Plasmid#198970PurposeLevel 0 partDepositorInsertPtetL
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_44 [hph]
Plasmid#198957PurposeLevel 0 partDepositorInserthph
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_33 [cat]
Plasmid#198946PurposeLevel 0 partDepositorInsertcat
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_99 [bsRBS]
Plasmid#199000PurposeLevel 0 partDepositorInsertbsRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_43 [tsr]
Plasmid#198956PurposeLevel 0 partDepositorInserttsr
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_78 [ORI_BC_3]
Plasmid#198979PurposeLevel 0 partDepositorInsertORI_BC_3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_88 [ORI_BC_13]
Plasmid#198989PurposeLevel 0 partDepositorInsertORI_BC_13
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_92 [ORI_BC_17]
Plasmid#198993PurposeLevel 0 partDepositorInsertORI_BC_17
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_83 [ORI_BC_8]
Plasmid#198984PurposeLevel 0 partDepositorInsertORI_BC_8
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_53 [TcatA]
Plasmid#198966PurposeLevel 0 partDepositorInsertTcatA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only